Fig. 5

Src expression is increased in breast carcinoma and inhibition of Src blocks cortisol-induced reactive nitrogen species. a Oncomine Cancer Microarray databases were used to analyse expression of SRC in the Curtis Breast database (n = 1600). Expression was compared between normal breast tissue (n = 144) and invasive breast carcinoma (n = 1456) (p = 1.46E-51). b MCF-7 cells were exposed to cortisol (Cort) (1 μM) for 20 minutes and to the glucocorticoid receptor (GR)-antagonist RU486 (1 μM) and Src inhibitor PP2 (10 μM) for 30 minutes. Cells were immunofluorescently labelled for the GR and counterstained with 4',6-diamidino-2-phenylindole. Representative images are shown. MCF-7 cells were incubated with cortisol (1 μM) (c, f), norepinephrine (NE) (1 μM) (d, g) or a combination of both (e, h) for 30 minutes after 30 minutes exposure to PP2 (10 μM). Levels of hydrogen peroxide (H ₂ O ₂ ) and nitrogen dioxide (NO ₂ ) were measured using electrochemical sensors. Results were analysed using analysis of variance (post hoc Tukey multiple comparisons). Data are mean ± SEM: *p < 0.05, **p < 0.01, ***p < 0.001