Fig. 1
Electrochemical analyses of reactive oxygen species/reactive nitrogen species produced in response to stress hormones over time. MCF-7, MDA-MB-231 and HCC38 cells were incubated with cortisol (1 μM) (a-c) and norepinephrine (1 μM) (d-f) for 15, 30 and 90 minutes. Cell lysates were collected and electrochemical were sensors used to measure levels of hydrogen peroxide (H 2 O 2 ), peroxynitrite (OONO -), nitric oxide (NO) and nitrogen dioxide (NO 2 ). Data are mean ± SEM and statistical significance was determined using one way analysis of variance (post hoc Tukey multiple comparisons). *Significant increase compared to baseline: *p < 0.05, **p < 0.01, ***p < 0.001