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Fig. 1 | Breast Cancer Research

Fig. 1

From: EZH2 protein expression in normal breast epithelium and risk of breast cancer: results from the Nurses’ Health Studies

Fig. 1

Imaging analysis of the normal breast tissue in the tissue microarrays (TMAs). a The pipeline used in this analysis consisted of the three basic stages: TMA core detection and identification matching the identification grid with the core location (upper panel); region of interest (ROI) segmentation, machine-learning-based training and application of the epithelium/stroma classifier (middle panel); and cell and nuclei detection and staining evaluation (bottom panel, left side). Using this pipeline, data were readily available for downstream analysis (bottom panel, right side - a detailed version of this panel is available in Additional file 1: Figure S2). b Representative images of immunohistochemical (IHC) expression of EZH2 in normal breast tissues. Scale bar 200 μm

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