Fig. 9
From: DCYTB is a predictor of outcome in breast cancer that functions via iron-independent mechanisms
Iron-responsive protein expression and cellular labile iron in response to DCYTB induction. a. Iron-responsive protein expression in T47D cells induced with doxycycline for 72 hours. Transferrin receptor and ferritin expression quantified with Fiji ImageJ [83] and normalized to uninduced cells containing vector alone. b Labile iron pool measurement of T47D cells induced with doxycycline for 72 hours and iron treated for 24 hours. c. Iron-respnsive proein expression in MCF7 cells treated as in panel (a). d. Labile iron pool measurements in MCF7 cells treated as in panel (b). Results represent the mean and standard deviation of at least 15 replicate samples. Similar results were obtained in at least three independent experiments. DCYTB duodenal cytochrome b