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Fig. 4 | Breast Cancer Research

Fig. 4

From: CCL2-driven inflammation increases mammary gland stromal density and cancer susceptibility in a transgenic mouse model

Fig. 4

The effect of CCL2 overexpression on mammary epithelial cell proliferation and cell death. Paraffin-embedded sections of mammary gland tissue from control and Mmtv-Ccl2 mice at proestrus were stained with anti-bromodeoxyuridine (BrdU) antibody to detect proliferating ductal (a and b) and alveolar (c and d) epithelial cells. The number of BrdU-positive cells (brown-stained cells) within ductal and alveolar epithelium was calculated and expressed as BrdU-positive cells/mm2 (i). Paraffin-embedded sections of mammary gland tissue from control and Mmtv-Ccl2 mice at proestrus were stained with TUNEL to detect dying ductal (e and f) and alveolar (g and h) epithelial cells. The percent of TUNEL-positive cells (green-stained cells) within ductal and alveolar epithelium was calculated (j); n = 6 per group. Data are presented as mean + SEM with statistical analysis conducted using the unpaired t test, *p < 0.05

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