Fig. 2
The effect of CCL2 overexpression on macrophage abundance and location within and around mammary epithelium. Paraffin sections of mammary gland tissue from control and Mmtv-Ccl2 were stained with anti-macrophage-specific F4/80 antibody to detect macrophages in the stroma surrounding ductal (a, b) and alveolar epithelium (c, d) of mammary glands from adult control and Mmtv-Ccl2 mice. Macrophages are indicated by black arrows. The number of F4/80-positive macrophages was quantified and represented as F4/80-positive macrophages/mm2 (e); n = 6 per group. Data are presented as mean + SEM with statistical analysis conducted using the unpaired t test, *p < 0.05