Fig. 3

Thymosin beta 10 (TMSB10) promotes the proliferation and tumorigenesis of breast cancer cells in vitro and in vivo. a In MTT assays, overexpression of TMSB10 significantly increased the growth rate of the indicated cells, while downregulation of endogenous TMSB10 significantly reduced the growth rate. Each bar represents the mean values ± SD of three independent experiments. b Overexpression of TMSB10 increased, while downregulation of endogenous TMSB10 reduced, the mean colony number in the colony formation assay. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. c Representative micrographs and colony numbers in the anchorage-independent growth assay. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. d Images of excised tumors from five BALB/c mice at 30 days after injection with the indicated cells. e Tumor volumes were measured every 5 days. Each bar represents the median values ± quartile values. f Average weight of excised tumors from the indicated mice. Each bar represents the median values ± quartile values. *P < 0.05