Skip to main content
Fig. 3 | Breast Cancer Research

Fig. 3

From: MCL-1 inhibition provides a new way to suppress breast cancer metastasis and increase sensitivity to dasatinib

Fig. 3

MCL-1 antagonism suppressed invasion and induced apoptosis of MDA-MB-231-2A cells in a 3D organotypic invasion assay. (a) Schematic of collagen I/fibroblast matrix contraction and (b) invasion assay with MDA-MB-231-2A cells invading through a contacted matrix towards a chemo-attractive gradient created by an air–liquid interface. Representative immunohistochemistry images of antibodies to multi-cytokeratin (c), Ki67 (e) and Cleaved Caspase 3 (g) on contracted matrices seeded with MDA-MB-231-2A cells treated with DOX or vehicle (Control) at day 1 and day 5 of invasion. Bar graphs depicting the average invasive index (d; total number of cells within the matrix expressed as a proportion of the number of cells on top with the opportunity to invade), the average proliferative index (f; ratio of Ki67-positive cells to total number of cells) and the average apoptotic index (h; ratio of Cleaved Caspase 3-positive cells to total number of cells). Average of three independent experiments. Bars indicate significantly significant groups, p value unpaired t tests. DOX doxycycline (Color figure online)

Back to article page