Fig. 6

Differential gene expression and response to 17β-estradiol (E2) in MDA-ERα46 and MDA-ERα66 cells. a The relative expression of a subset of genes was evaluated in MDA, MDA-ERα46, and MDA-ERα66 cells by quantitative RT-PCR following a 4-h treatment with E2 or ethanol as vehicle (V). Data were normalized to those obtained for the Rplp0 gene. Results shown within the heatmap are expressed as the mean log2 of these expression values normalized to those obtained in the control MDA cell line. b. Significant gene regulations by E2 in MDA-ERα46 and MDA-ERα66 cells or significant different basal gene expression between naïve MDA cells and estrogen receptor (ER) isoforms expressing cells are shown as heatmaps of P values. c. Examples of gene expression data obtained on six genes