Fig. 2
From: The AF-1-deficient estrogen receptor ERα46 isoform is frequently expressed in human breast tumors
Evaluation of the relative expression of estrogen receptor alpha (ERα)46 and ERα66 in human ERα-positive breast tumors. a Representative image of Western blot of human ERα-positive breast tumor samples blotted with an anti-ERα antibody (SP1). One result representative of an ERα-negative tumor is also shown. The MDA-ER66 cell line that co-expresses ERα66 and ERα46 was used as a positive control. The ERα46/ERα66 expression ratio is indicated below each lane. b The summarized data expressed as percentages of tumors expressing ERα46 or not. c Distribution of the ERα46/ERα66 expression ratio among the 116 tumor samples. d The number and grade of tumors expressing ERα46. e The ERα46/ERα66 expression ratio depending upon tumor grade (I, II, or III). f, g The ERα46/ERα66 expression ratio in correlation to tumor size. The whiskers in the boxplots indicate the 10–25% and 75–90% intervals