Fig. 1

Nuclear localization of ADA3 in immortal and estrogen receptor positive breast cancer cell lines. a Western blot analysis of ADA3 expression in immortal hMECs (76 N-TERT, MCF10A) and ER+ breast cancer cell lines (MCF-7, T47D, ZR-75-1). b ADA3 immunofluorescence staining in MCF-7 and 76 N-TERT cell lines, DAPI was used for nuclear staining. c Nuclear and cytoplasmic fractions were prepared from normal mammary epithelial cell (76 N), immortal mammary epithelial cells (76 N-TERT and MCF10A), and ER+ breast cancer cell lines (MCF-7, ZR-75-1). Protein concentration was quantitated, equal amounts of protein were separated on SDS-PAGE gel and then expression of various protein was assessed with indicated antibodies. PARP was used as a nuclear control, and GAPDH was used as a cytoplasm control