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Fig. 1 | Breast Cancer Research

Fig. 1

From: Evidence of two distinct functionally specialized fibroblast lineages in breast stroma

Fig. 1

Characterization, isolation and cultivation of interlobular and intralobular fibroblasts. a Multicolor imaging of cryostat sections of normal breast tissue showing an interlobular duct (left) and a terminal duct lobular unit (TDLU, right) stained for CD105 (green), CD26 (red) and nuclei (blue). An intralobular terminal duct (ITD) connects the TDLU to the interlobular duct. Phenotypically distinct fibroblasts surround the two anatomical structures. b Fluorescence activated cell sorting diagram of serially passaged fibroblasts stained with CD26 and CD105. Circles indicate gates selected for sorting. c Time course of population doublings of CD26high (light squares) and CD105high (dark squares) fibroblasts in serial passage subculture (scale bar = 50 μm)

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