Fig. 5
Protein tyrosine kinase C theta isoform (PRKCQ) downregulation inhibits growth of triple-negative breast cancer (TNBC) cells in culture and triple-negative breast tumor xenografts. a Lentiviral PRKCQ short hairpin (sh)RNA vector-infected TNBC cells were lysed and probed for expression of protein kinase C (PKC) family members. b TNBC cells expressing empty vector control (EV) or PRKCQ shRNA (90, 54 or 16) were grown in monolayer cultures for the indicated number of days and counted. c TNBC cells expressing vector control or PRKCQ shRNA (90, 54 or 16) were cultured in suspension for 24 hours. Cell death (anoikis) was assessed using the Cell Death ELISA. d TNBC cells expressing EV control or PRKCQ shRNA vectors were cultured in 3-D MatrigelTM cultures in chamber slides for the indicated number of days. e MDA-231-Luc-D3H2LN cells (5 × 105) expressing vector control or PRKCQ shRNA (90 or 54) were injected subcutaneously into the flank of 6-week-old, female nude mice. Tumor size was measured and recorded every 3–4 days. All figures are representative of three independent experiments except for the xenograft study, which was performed twice. Scale bars represent 200 μM. GAPDH glyceraldehyde-3-phosphate dehydrogenase