Skip to main content
Fig. 2 | Breast Cancer Research

Fig. 2

From: WNT4 mediates estrogen receptor signaling and endocrine resistance in invasive lobular carcinoma cell lines

Fig. 2

Estrogen regulation of WNT4 correlates with estrogen receptor (ER) binding at the WNT4 estrogen receptor binding site (ERBS). a Breast cancer cell lines (BCCLs) were hormone-deprived and treated in biological triplicate with vehicle (0.2 % EtOH), 1 nM 17β-estradiol (E2), 100 nM progesterone (P4), 1 μM ICI 182,780 (fulvestrant; ICI), or 1 μM RU486 (RU), as indicated. RNA was harvested 24 h posttreatment. Bars represent mean ± SD as fold change vs vehicle control; red error bars indicate analysis of variance (Dunnett’s multiple comparisons test) vs vehicle control (p < 0.05). b Data from (a) were normalized to a pan-average of all samples across BCCLs tested. c BCCLs were hormone-deprived and treated as indicated for 45 minutes. Chromatin immunoprecipitation (ChIP) was performed as described in the Methods section. Red values indicate fold enrichment for E2 vs vehicle in ER ChIP. The data were derived from a single experiment but are representative of two or three experiments. SUM44 SUM44PE cell line, HR Hormone receptor, IDC Invasive ductal carcinoma, IgG Immunoglobulin G, ILC Invasive lobular carcinoma, PR Progesterone receptor

Back to article page
\