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Fig. 8 | Breast Cancer Research

Fig. 8

From: CUB domain-containing protein 1 and the epidermal growth factor receptor cooperate to induce cell detachment

Fig. 8

Enigma/PDLIM7 as a novel CDCP1-associated protein. a MDA-MB-231 cells were transduced with the indicated recombinant adenoviruses, then treated for 15 minutes with 100 μM sodium pervanadate just before cell lysates were prepared. The various forms of CDCP1 were isolated by anti-FLAG affinity chromatography and CDCP1-associated proteins were identified by immunoblot. Note that the phospho-PKCδ[Y311] antibody cross-reacts with phosphorylated Tyr707 of CDCP1 and that staining was absent when the Y707F mutant of CDCP1 was employed. b HEK 293 cells were transfected with the indicated constructs, cell lysates were immunoprecipitated with an E-cadherin antibody, and the immunoprecipitates were analyzed by immunoblot. c HEK 293 cells were transfected with the indicated constructs, cell lysates were immunoprecipitated with anti-FLAG agarose, and the immunoprecipitates were analyzed by immunoblot. d HEK 293 cells were transfected with the indicated constructs, cell lysates were immunoprecipitated with an E-cadherin antibody, and the immunoprecipitates were analyzed by immunoblot

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