Fig. 7

CDCP1 complexes are similar between breast and pancreatic cancer cell lines and are stable in a variety of detergents. a MDA-MB-231 or T47D cells were transduced with the indicated adenoviral vectors, CDCP1-containing complexes were isolated with anti-FLAG agarose, and the presence of components of adherens and desmosomal junctions was assessed by immunoblot analysis. b This study was carried out similarly to the study in Fig. 6a, except that the pancreatic cancer cell lines AsPC1 or PANC1 were used. c AsPC1 cells were transduced with control (GFP) or ΔCDCP1 adenoviruses, the cells were extracted with the indicated detergents, and ΔCDCP1-associated proteins were examined by immunoblot (left panel) or total protein levels were assessed by Coomassie staining of the same samples resolved by SDS-PAGE (right panel)