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Fig. 4 | Breast Cancer Research

Fig. 4

From: CUB domain-containing protein 1 and the epidermal growth factor receptor cooperate to induce cell detachment

Fig. 4

CDCP1, EGFR, and EGF cooperate to induce E-cadherin internalization. a T47D cells stably expressing an E-cadherin-GFP fusion protein and either EGFR, CDCP1, or EGFR + CDCP1, or the corresponding vector control line were plated on glass coverslips coated with collagen I and treated for 24 hours with or without 10 ng/ml EGF. Intracellular E-cadherin-GFP localization was imaged by confocal immunofluorescence microscopy. b The indicated T47D cell lines were stimulated with or without 20 ng/ml EGF for 24 hours and subjected to cell surface biotinylation. Biotinylated proteins (external, Ext.) were isolated by streptavidin-agarose chromatography and unbound proteins were collected as non-biotinylated proteins (internal, Int.). Protein fractions were analyzed by immunoblot. c The indicated cell lines treated as in Fig. 4b were extracted with 1 % Triton X100 and analyzed by immunoblot

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