Fig. 1

CDCP1 mediates suspension growth of MDA-MB-468 cells. a Morphology of the MDA-MB-468 vector control cell line or a line stably overexpressing wild-type CDCP1. CDCP1 induces suspension growth of cell cultures. b Comparison of MDA-MB-468 cell lines expressing wild-type CDCP1, or a phospho-defective form of CDCP1 in which tyrosine residues 707, 734, and 762 have been mutated to phenylalanine ([3YF]), grown in either soft agar or collagen I gels. Note that CDCP1-dependent suspension growth requires tyrosine phosphorylation. c Immunoblot analysis showing that the MDA-MB-468 cell lines stably express CDCP1 or its mutants at similar levels, but only wild-type CDCP1 increases activating Src phosphorylation above basal levels. d Treatment of MDA-MB-468 cells with Disulfide bond Disrupting Agents (DDAs) induces parallel mobility shifts of EGFR and CDCP1 in time- and concentration-dependent manners