Skip to main content
Fig. 8 | Breast Cancer Research

Fig. 8

From: SMARCE1 regulates metastatic potential of breast cancer cells through the HIF1A/PTK2 pathway

Fig. 8

HIF1A activates PTK2 transcription and downstream survival pathways in detached cells. a Detachment induced HIF1A protein accumulation is eliminated by HIF1A knockdown in MDA-MB-231 cells. b HIF1A knockdown abolishes detachment induced PTK2 mRNA expression in MDA-MB-231 cells. c HIF1A knockdown sensitizes MDA-MB-231 cells to anoikis. Viable cells in suspension culture for 10 days were counted. d Effect of HIF1A knockdown in MDA-MB-231 cells on detachment-induced activation of pro-survival (PTK2, ERK, and AKT) and pro-apoptotic (BIM) signaling pathways. e Effect of PTK2 inhibition in MDA-MB-231 cells on detachment-induced activation of pro-survival and pro-apoptotic signaling pathways. The mRNA levels were measured by qPCR. The levels of protein expression and phosphorylation were determined by immunoblotting assays with GAPDH included as a loading control. *p < 0.05, Student t test. EV empty vector, KD knockdown, LM lung metastatic cell line derived from MDA-MB-231

Back to article page