Fig. 4

IGF1R-KD induces SLPI downregulation and CTGF upregulation in CD24⁺. SLPI and CTGF mRNA levels were determined by qRT-PCR analysis in WT (a) and MKR (b) tumors. Tumor lysates from WT (c) and MKR (e) mice were separated by SDS-PAGE, and SLPI levels were assessed using Western blotting. d, f Relative expression was quantified by densitometric analysis and is presented as a fold change compared with the control group. Equal loading of proteins was demonstrated by immunoblotting with an antibody directed against β-actin. g, h qRT-PCR analysis of SLPI and CTGF in vitro. Mann-Whitney test was performed to compare the difference between the control group and IGF1R-KD group, *P < 0.05, **P < 0.005, ***P < 0.001. CTGF connective tissue growth factor, IGF1R insulin-like growth factor receptor, KD knockdown, mRNA messenger RNA, SLPI secretory leukocyte protease inhibitor