Fig. 3

Knockdown of IGF1R in CD24⁺ Mvt1 cells impairs mammary tumor formation. Cells were injected into the mammary fat pad of 8-week-old WT and MKR female mice. Tumor volume (a, b) was monitored during 4.5 weeks and tumor weights (c, d) were measured at necropsy. Tumor lysates from WT (e) and MKR (g) mice were separated by SDS-PAGE, and IGF1R levels were assessed using Western blotting. f, h Relative expression was quantified by densitometric analysis and is presented as a fold change compared with the control group. Equal loading of proteins was demonstrated by immunoblotting with an antibody directed against β-actin. Mann-Whitney test was performed to compare the difference between the control tumors and IGF1R-KD tumors, *P < 0.05, and difference between WT and MKR tumors ^# P < 0.05. IGF1R insulin-like growth factor receptor, KD knockdown