Fig. 2

IGF1R-KD has a profound effect on CD24⁺ cells morphology and phenotype. a FACS histograms of control and IGF1R-KD Mvt-1 cells following sorting into pure CD24^- and CD24⁺ cell populations. b Cell phenotype in adherent culture for each group is presented in a phase-contrast bright field image. c Representative photomicrographs of tumorsphere (> 50 um diameter) grown from single cells in nonadherent culture for 5 days (upper panel). TFE (%) comparison between the groups (lower panel). IGF1R insulin-like growth factor receptor, KD knockdown, TFE tumorsphere formation efficiency