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Fig. 1 | Breast Cancer Research

Fig. 1

From: Increased chromosomal radiosensitivity in asymptomatic carriers of a heterozygous BRCA1 mutation

Fig. 1

Design of the G2 micronucleus (MN) assay and overview of the cell cycle. a Left: Day 0: setup of one 50-ml large blood culture for every patient. Day 3: division of the cultures and subsequent irradiation. Addition of cytochalasin B and caffeine (CAF; if needed) immediately after irradiation, followed by incubation during 8 h before harvesting. Right: 4′,6-diamidino-2-phenylindole-stained binucleated cell with micronucleus generated with the Metafer4 system (MetaSystems). b Overview of the cell cycle for proliferating lymphocytes and approximate duration of the different phases with indication of the applied post-irradiation incubation period (black). At the start of irradiation, all lymphocytes in the blood culture were mononucleated. After a post-irradiation incubation of 8 h, the cultures were fixed and binucleated (BN) cells (cells that went through one mitosis) were scored for the presence of MN

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