Fig. 7

Ectopic expression of miR-720 restores the migratory and invasive abilities of MDA-MB-231 cells with stable knockdown of ADAM8. MDA-MB-231 shA8-20 clonal cells, with stable ADAM8 knockdown, were transfected in six-well plates with 200 pmol of control mimic (Ctrl) or miR-720 mimic. After 48 h, either RNAs were isolated or cells subjected to functional assays. a miR-720 levels were determined by RT-qPCR. The control mimic (Ctrl) was set to 1 (mean ± SD from three independent experiments). b Transfected MDA-MB-231 shA8-20 cells were plated in Matrigel and photographed at 10× magnification after 11–15 days of culture. Representative images of two independent experiments with similar results are shown. Scale bar = 100 μm. c Transfected cells were subjected to a Boyden chamber migration assay for 24 h, as in Fig. 5. Ctrl was set to 100 % (mean ± SD from three independent experiments). d Transfected cells were subjected to an ATP assay following growth on plastic for 24 h. Ctrl was set to 100 % (mean ± SD from three independent experiments). **P < 0.01, ***P < 0.001, Student’s t test. Not sig. not significant, Rel. relative