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Fig. 6 | Breast Cancer Research

Fig. 6

From: miR-720 is a downstream target of an ADAM8-induced ERK signaling cascade that promotes the migratory and invasive phenotype of triple-negative breast cancer cells

Fig. 6

miR-720 maintains the migratory and invasive properties of SUM-149 cells. SUM-149 cells were transfected with 50 nM anti-miR-720 or anti-miR-negative control (Ctrl) for 48 h, and either RNA isolated or cells subjected to functional assays. a miR-720 levels were determined using RT-qPCR, as in Fig. 5a. b Migration assay. Transfected cells that migrated through a Boyden chamber over a 24-h period were quantified as in Fig. 5b. Ctrl was set to 100 % (mean ± SD from three independent experiments). c Invasion assay. Transfected cells that invaded through a Matrigel-coated Boyden chamber in a 12-h period were quantified. Ctrl was set to 100 % (mean ± SD from three independent experiments). d ATP assay. Transfected cells were grown for 24 h and ATP levels measured using an ATPlite 1Step assay. Ctrl was set to 100 % (mean ± SD from three independent experiments). **P < 0.01, ***P < 0.001, Student’s t test. Not sig. not significant, Rel. relative

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Breast Cancer Research

ISSN: 1465-542X

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