Fig. 2

Naringenin (Nar) prevents pulmonary metastasis derived from the primary breast tumor. a Bioluminescence imaging of pulmonary metastasis in vivo. Mice bearing 4T1 tumor were treated with Nar or 1D11 for 34 days and then were imaged with bags to avoid the bioluminescence from primary tumor. b Bioluminescent photons of the pulmonary area from the mice treated with Nar or 1D11 antibodies for 34 days measured using the IVIS system (median ± IQ range). c Survival of mice bearing 4T1/RFP tumor (left) or 4T1/TGF-β1 (right) tumor after treatment with Nar or 1D11 antibodies. d TGF-β1 concentration in lung homogenates and serum. Results are representative of three experiments. *P <0.05, **P <0.01, ***P <0.001. Error bars indicate SE. TGF transforming growth factor