Fig. 3
From: Activation of IGF1R/p110β/AKT/mTOR confers resistance to α-specific PI3K inhibition
BYL719 resistance of breast cancer cell lines is dependent on IGF1R activity. a BYL719 dose-responses of parental and resistant lines after 3 days of treatment in the presence or absence of insulin in the culture media. Cell numbers were evaluated using the sulforhodamide B assay. GI50 (50 % growth inhibition) values were calculated using GraphPad Prism 6 software. Data are mean ± SEM (n = 3). b Proliferation of parental and resistant cells treated with vehicle (VHC), AEW541 (1 μM), BYL719 (IC90), or a combination of BYL719 and AEW541 for 9 days. Cell numbers were evaluated using the sulforhodamide B assay. Data are mean ± SEM (n >3, *P <0.01, **P <0.001). c Immunoblots of lysates from parental and resistant cells treated for 24 hours as indicated. BYL719 (IC90) and AEW541 (1 μM). MCF7p MCF7-parental, MCF7r MCF7-resistant, T47Dp T47D-parental, T47Dr T47D-resistant