Fig. 4

Gene expression of cell isolates. Real time PCR results show gene expression (n = 48) genes, including a house keeping gene after comparison to the mean expression of all normal (NORMA1-4) respective MEC, MES or ADSC primary cell lines. a Expression of positive stem cell markers CD105, CD73, CD90, CD36, CD29, CD44 and of vimentin (VIM) (ADSC=1) and hematopoietic markers CD14, CD19, CD34, CD45, HLA-DR are shown (PBC/BEC=1). b Panel shows invasion and EMT marker genes, MMP2, MMP9 and SNAI1, the fibroblast mesenchymal marker S100A4 and N-cadherin (CDH2) and CDH11, markers of the mesenchymal phenotype (MES=1). c Epithelial cell markers (MEC=1) compared to ADSC and MES. Specific markers include epithelial stem cell markers CD24 and CD49f and markers of basal myoepithelial mammary cells (ACTA1, CNN1, ACTA2, CD10). d Six endothelial marker genes analyzed were PECAM1, VWF, FLT1, KDR, FLT4, CDH5 and PDGFRB (BEC=1). Significant values compared with BEC (^*, ^**) or MEC NORMA1 (^#, ^##). (^*,^# p ≤ 0.05, ^**,^## p ≤ 0.01) (ADSC = adipose derived stem cells, BEC = blood endothelial cells MEC = mammary epithelial cells, MES = mesenchymal cells, PBC = peripheral blood cells)