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Fig. 2 | Breast Cancer Research

Fig. 2

From: In patients with metastatic breast cancer the identification of circulating tumor cells in epithelial-to-mesenchymal transition is associated with a poor prognosis

Fig. 2

Enumeration and sorting of viable single CTC by DEPArray technology. a Image gallery of MDA-MB231 and MCF-7 cells, analyzed from a spiked sample, showing, from top to bottom, two cells coexpressing mesenchymal (red fluorescence) and epithelial (green fluorescence) markers, one cell expressing only mesenchymal markers, and one cell expressing only epithelial markers, respectively. Images of tumor cell morphology are shown in the bright-field channel (grey), while cell nuclei are stained by Hoechst (blue). b Real-time PCR showing cytokeratin-19 and vimentin expression in sorted, single MCF-7 (green line) and MDA-MB231 (red line) cells. c Representative images of the analysis of a blood sample from a MBC patient enriched employing the strategy described in Fig. 1a. The CD45-depleted fraction was labeled with antibodies recognizing epithelial (green fluorescence) and mesenchymal (red fluorescence) markers, as well as the common leukocyte antigen CD45 (cyan fluorescence). The image bar displays, from top to bottom, one cell expressing only epithelial markers (E CTC), one cell coexpressing mesenchymal and epithelial markers (EM CTC), one cell expressing only mesenchymal markers (MES), one CD45-positive leukocyte (LEUK), and one cell negative for all of the tested markers (NEG), respectively. Images of tumor cell morphology are shown in the bright-field channel (grey), while cell nuclei are stained by Hoechst (blue). d Real-time PCR showing cytokeratin-19, vimentin, HER2, and ER expression in sorted, single E-CTC (green line), EM-CTC (red line), and MES (blue line) cells (Color figure online)

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