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Fig. 3 | Breast Cancer Research

Fig. 3

From: Interferon-induced transmembrane protein 1 (IFITM1) overexpression enhances the aggressive phenotype of SUM149 inflammatory breast cancer cells in a signal transducer and activator of transcription 2 (STAT2)-dependent manner

Fig. 3

Effects of short hairpin RNA (shRNA) knockdown of interferon-induced transmembrane protein 1 (shIFITM1) on cell proliferation, migration, and colony formation in SUM149 cells. a Western blot analysis was used to confirm the shRNA knockdown of IFITM1 in SUM149 cells. b Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in SUM149 cells that were transfected with shRNA for 24 h and maintained in culture for a further 48 h. The assay results showed that the knockdown of IFITM1 reduced cell proliferation by up to 52 % in SUM149 cells compared with the short hairpin control cells (shCon). c Cell migration was assessed by wound-healing assay. d Left panel: 2-D colonies formed by SUM149 cells with and without shRNA knockdown. Right panel: Quantification of the number of colonies. *P < 0.05

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