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Fig. 1 | Breast Cancer Research

Fig. 1

From: Interferon-induced transmembrane protein 1 (IFITM1) overexpression enhances the aggressive phenotype of SUM149 inflammatory breast cancer cells in a signal transducer and activator of transcription 2 (STAT2)-dependent manner

Fig. 1

Interferon-induced transmembrane protein 1 (IFITM1) protein and messenger RNA (mRNA) expression in breast cancer cells. a IFITM1 protein expression in the triple-negative SUM149 cells, human epidermal growth factor receptor 2–overexpressing SUM190 and MDA-IBC-3 cells, and non–inflammatory breast cancer (non-IBC) MCF-7 cells. The protein expression was assessed by Western blot analysis, with β-actin used as a loading control. b IFITM1 mRNA expression in SUM149 and SUM190 cells. The mRNA levels were determined by real-time polymerase chain reaction, and the fold change for each cell line was calculated against the Pumilio RNA-binding family member 1 (PUM1) internal control gene. Each value shown is the mean ± standard deviation from three independent experiments. *P < 0.05. c IFITM1 protein expression and localization profiles of SUM149 and SUM190 IBC cells determined by immunofluorescence. The images of the cells were captured using a Leica confocal microscope equipped with Leica Application Suite Advanced Fluorescence Lite 2.6.0 Build 7266 software. DAPI 4′,6-diamidino-2-phenylindole

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Breast Cancer Research

ISSN: 1465-542X

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