Fig. 3

Growth pattern of hMECs with IR treatment and time. a Schematic representation of the multi-dimensional analysis performed with high-content screening. The different variables assessable by our experimental design are given, where axis length represents magnitude of variation and/or classification possible within the scope of this analysis. b Schematic representation of the variables simultaneously captured for a given population on a cell-by-cell basis using high-content screening. c Average number of cells by time and increasing IR dose for four donors. Mean total number of cells over (n = 5–6) technical replicates (± SEM) over 48 hours with 5 Gy IR treatment (dashed line) compared with controls (solid line, 0 Gy). d Response of hMECs to increasing dose of IR. Mean total number of cells (± SEM) over 48 hours with 0, 1, 2 and 5 Gy levels of IR for donor Q687. e Estimated growth pattern based on technical replicates of hMECs with time and dose stratified by cell phenotype or subpopulation. Estimated subpopulation (± SEM at fixed observation times) over 48 hours with and without treatment (5 and 0 Gy, respectively) plotted for donor Q687. DAPI 4′,6-diamidino-2-phenylindole, DN, double negative, DP double positive, K14 cytokeratin 14, K19 cytokeratin 19