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Fig. 2 | Breast Cancer Research

Fig. 2

From: High content screening application for cell-type specific behaviour in heterogeneous primary breast epithelial subpopulations

Fig. 2

Assessment of alternative markers to delineate hMEC subpopulations using high-content screening. a Immunofluorescence staining of hMECs with DAPI (white) and antibodies against K14 (red), K19 (blue) and K8/18 (green; top panel) or K5 (green; bottom panel). Images acquired at 20× magnification using INCA. b Proportional Venn diagrams depict the mean overall proportions and overlap of K14, K8/18 and K19 expression (top) and K14, K5 and K19 expression (bottom) in first-passage hMEC cultures for donor Q626. c IHC staining of K5 and K8/18 on FFPE sections of normal human breast. Staining for K5 (top left, 10×) delineates both basal cells (i; top right, 20×) and luminal cells (ii; bottom left, 20×). Staining for K8/18 (bottom right, 10×) preferentially delineates basal cells. DAPI 4′,6-diamidino-2-phenylindole, K5 cytokeratin 5, K8/18 cytokeratin 8/18, K14 cytokeratin 14, K19 cytokeratin 19

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Breast Cancer Research

ISSN: 1465-542X

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