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Table 1 Percentages of apoptotic cells following a 72-h epirubicin treatment

From: Downregulation of histone H2A and H2B pathways is associated with anthracycline sensitivity in breast cancer

Cell line Control Epirubicin concentration
MDA-MB-231 DMSO 1 nM 25 nM 1000 nM
Native 18 % 17 % 41 % 94 %
25 nM-R 10 % 10 % 8 % 50 %
MCF7 DMSO 1 nM 30 nM 1000 nM
Native 32 % 29 % 49 % 77 %
30 nM-R 20 % 24 % 23 % 78 %
SKBR3 DMSO 1 nM 15 nM 1000 nM
Native 22 % 26 % 24 % 59 %
15 nM-R 18 % 17 % 17 % 34 %
ZR-72-1 DMSO 1 nM 10 nM 1000 nM
Native 36 % 44 % 47 % 71 %
10 nM-R 29 % 28 % 29 % 62 %
  1. DMSO dimethyl sulphoxide; DRFS, distant relapse-free survival
  2. Apoptotic cells = annexin V–positive. Debris and necrotic cells (annexin V–negative, 7-aminoactinomycin D–positive) were gated out. Percentages reported are derived from a single experiment; at least two independent experiments were done for each cell line
  3. Bold data denotes the different concentrations of epirubicin used for each cell line