|
Cell line
|
Control
|
Epirubicin concentration
|
|---|
|
MDA-MB-231
|
DMSO
|
1 nM
|
25 nM
|
1000 nM
|
|
Native
|
18 %
|
17 %
|
41 %
|
94 %
|
|
25 nM-R
|
10 %
|
10 %
|
8 %
|
50 %
|
|
MCF7
|
DMSO
|
1 nM
|
30 nM
|
1000 nM
|
|
Native
|
32 %
|
29 %
|
49 %
|
77 %
|
|
30 nM-R
|
20 %
|
24 %
|
23 %
|
78 %
|
|
SKBR3
|
DMSO
|
1 nM
|
15 nM
|
1000 nM
|
|
Native
|
22 %
|
26 %
|
24 %
|
59 %
|
|
15 nM-R
|
18 %
|
17 %
|
17 %
|
34 %
|
|
ZR-72-1
|
DMSO
|
1 nM
|
10 nM
|
1000 nM
|
|
Native
|
36 %
|
44 %
|
47 %
|
71 %
|
|
10 nM-R
|
29 %
|
28 %
|
29 %
|
62 %
|
-
DMSO dimethyl sulphoxide; DRFS, distant relapse-free survival
- Apoptotic cells = annexin V–positive. Debris and necrotic cells (annexin V–negative, 7-aminoactinomycin D–positive) were gated out. Percentages reported are derived from a single experiment; at least two independent experiments were done for each cell line
- Bold data denotes the different concentrations of epirubicin used for each cell line
