Fig. 6

Effect of Src homology phosphotyrosyl phosphatase 2 (SHP2) silencing on fibroblast growth factor receptor (FGFR) and c-Met expression and signaling. a Effect of SHP2 silencing on fibroblast growth factor (FGF)-induced signaling in the MDA-MB-231 cells. b Effect of SHP2 silencing on FGF-induced signaling in the MDA-MB-468 cells. c Effect of SHP2 silencing on hepatocyte growth factor (HGF)-induced signaling in the MDA-MB-231 cells. d Effect of SHP2 silencing on HGF-induced signaling in the MDA-MB-468 cells. Note that silencing SHP2 expression led to suboptimal and short-lived FGF- or HGF-induced extracellular signal-regulated kinases 1 and 2 (ERK1/2) and Akt activation in both cell lines. e Effect of SHP2 silencing on the protein levels of FGFR1 and c-Met in the MDA-MB-231 and MDA-MB-468 cells. f Average of band density measurements for FGFR and c-MET from three independent experiments. Con controls, sh-1 SHP2 short hairpin RNA-1, sh-2 SHP2 short hairpin RNA-2