Fig. 5

Effect of Src homology phosphotyrosyl phosphatase 2 (SHP2) and epidermal growth factor receptor (EGFR) silencing on cell proliferation, anchorage-independent growth, and mammosphere formation. a Immunoblotting analysis of EGFR silencing with two independent short hairpin RNA (shRNA) constructs in the MDA-MB-231 and MDA-MB-468 cells. b Effect of EGFR and SHP2 silencing on cell proliferation in MDA-MB-231 cells. c Effect of EGFR and SHP2 silencing on cell proliferation in MDA-MB-468 cells. d Effect of EGFR and SHP2 silencing on anchorage-independent growth in soft agar. e Colony numbers from three independent experiments in MDA-MB-231 cells cultured as shown in (d). f Colony numbers from three independent experiments in MDA-MB-468 cells cultured as shown in (d). g Effect of EGFR and SHP2 silencing on mammosphere formation in MDA-MB-231 cells. h Effect of EGFR and SHP2 silencing on proportion of aldehyde dehydrogenase 1 (ALDO)-high cells in MDA-MB-231 cells. P parental, C control, sh-1 SHP2 shRNA-1, sh-2 SHP2 shRNA-2, Esh1 EGFR shRNA-1, Esh2 EGFR shRNA-2, SSC side scatter