Fig. 2
From: HDAC6 activity is a non-oncogene addiction hub for inflammatory breast cancers
Validation of histone deacetylase 6 (HDAC6) as a positive screen hit. a The western blot shows the efficiency of two independent shRNAs in silencing HDAC6 in different breast cancer cell lines. The numbers below the blots indicate the fraction of protein remained normalized to β-Actin and to the amount detected in cells expressing scrambled shRNA. b Graphic representation of Annexin-V/7-AAD assay to measure the induction of apoptosis when HDAC6 is silenced by the shRNAs shown in a. Late apoptosis and early apoptosis are combined. c Illustrative example of the FACS data obtained from the Annexin-V/7-AAD assay. d Cell numbers after several doubling times in inflammatory breast cancer (IBC) and non-IBC cell lines when HDAC6 was knocked down (shRNA#1). The data are normalized to the scrambled shRNA control