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Table 1 Antibodies and siRNAs

From: Dual inhibition of Type I and Type III PI3 kinases increases tumor cell apoptosis in HER2+ breast cancers

Antibody Vendor Catalog number Dilution siRNA Vendor Catalog number
p110α Cell Signaling 4249 1:1,000 Universal neg ctrl Sigma SIC003
p110β Cell Signaling 3011 1:1,000 p110α-silencing Sigma SASI_Hs01_00219338
P-Akt (S473) Cell Signaling 9271 1:1,000 p110α-silencing Sigma SASI_Hs01_00219339
Akt Cell Signaling 9272 1:2,000 Vps34-silencing Sigma SASI_Hs01_00233716
P-S6 (S235/6) Cell Signaling 4858 1:1,000 Vps34-silencing Sigma SASI_Hs02_00334179
S6 Cell Signaling 2317 1:1,000    
P-HER2 (Y1221) Cell Signaling 2243 1:1,000    
HER2 Thermo Rb103 1:1,000    
Vps34 Cell Signaling 3358 1:1,000    
LC3 Cell Signaling 4108 1:1,000    
Actin Cell Signaling 4970 1:5,000    
PARP Cell Signaling 9532 1:1,000    
Cleaved caspase 3 Cell Signaling 9661 1:500    
  1. HER2 human epidermal growth factor receptor-2, PARP poly(ADP-ribose) polymerase Cell Signaling Technologies (Danvers, MA, USA). Thermo-Fisher (Waltham, MA, USA). Sigma-Aldrich (St. Louis, MO, USA)