Dual inhibition of Type I and Type III PI3 kinases increases tumor cell apoptosis in HER2+ breast cancers

Breast Cancer Research

Table 1 Antibodies and siRNAs

Antibody	Vendor	Catalog number	Dilution	siRNA	Vendor	Catalog number
p110α	Cell Signaling	4249	1:1,000	Universal neg ctrl	Sigma	SIC003
p110β	Cell Signaling	3011	1:1,000	p110α-silencing	Sigma	SASI_Hs01_00219338
P-Akt (S473)	Cell Signaling	9271	1:1,000	p110α-silencing	Sigma	SASI_Hs01_00219339
Akt	Cell Signaling	9272	1:2,000	Vps34-silencing	Sigma	SASI_Hs01_00233716
P-S6 (S235/6)	Cell Signaling	4858	1:1,000	Vps34-silencing	Sigma	SASI_Hs02_00334179
S6	Cell Signaling	2317	1:1,000
P-HER2 (Y1221)	Cell Signaling	2243	1:1,000
HER2	Thermo	Rb103	1:1,000
Vps34	Cell Signaling	3358	1:1,000
LC3	Cell Signaling	4108	1:1,000
Actin	Cell Signaling	4970	1:5,000
PARP	Cell Signaling	9532	1:1,000
Cleaved caspase 3	Cell Signaling	9661	1:500

HER2 human epidermal growth factor receptor-2, PARP poly(ADP-ribose) polymerase Cell Signaling Technologies (Danvers, MA, USA). Thermo-Fisher (Waltham, MA, USA). Sigma-Aldrich (St. Louis, MO, USA)

ISSN: 1465-542X