Fig. 1
From: β2-adrenoceptor signaling regulates invadopodia formation to enhance tumor cell invasion
Breast cancer cells have functional β2-adrenoceptors (β 2 AR). a ADRB1, ADRB2, and ADRB3 mRNA transcript levels were quantified by quantitative RT-PCR, with expression normalized to ACTB. b Immunostaining of β2AR and macrophages in archival breast cancer tissue. Scale bar: 100 μm. c, d cAMP accumulation was quantified in c 66cl4 cells and d MDA-MB-231 cells after treatment with vehicle, or 10 nM isoproterenol ± 1 μM antagonists including propranolol (nonselective), CGP-20712A (β1AR selective), ICI-118551 (β2AR selective), or L748337 (β3AR selective). N = 3. Error bars: standard error of the mean (SEM). ND: not detected. *p <0.05 and **p <0.01