Fig. 1

Identification of differentially regulated cell surface markers during chemotherapeutic treatment. a Study design for chemotherapy treatment of xenograft tumors, blue circles around graphed points represent time points of analysis . b Immunohistochemical analysis of sialyl-glycolipid stage-specific embryonic antigen 4 (SSEA4) expression (red, SSEA4; blue, 4′,6-diamidino-2-phenylindole). c, d The expression patterns of cell surface markers in xenograft tumors from four individual breast cancer patients were analyzed by multiparametric flow cytometry during the course of treatment. e The expression of SSEA4 during chemotherapeutic treatment of xenograft tumors was analyzed at four time points (n = 8). ***p < 0.001; scale bars = 300 μm for larger images and 100 μm for insets. A/C doxorubicin/cyclophosphamide, HBCx human breast cancer xenograft, TGFβR transforming growth factor β receptor