Puberty-specific promotion of mammary tumorigenesis by a high animal fat diet

Breast Cancer Research

Table 4 RT-PCR analysis of gene expression at 13 weeks old with DMBA treatment

		Fold regulation (compared with LFD)
Symbol	Description	HFD	LFD-HFD	HFD-LFD
Tgfa	Transforming growth factor alpha	2.4	2.1	2.0
Ccl1	Chemokine (C-C motif) ligand 1	3.8	3.2	NS
Ccl17	Chemokine (C-C motif) ligand 17	2.8	4.0	NS
Ccl20	Chemokine (C-C motif) ligand 20	11.0	5.0	NS
Ccl22	Chemokine (C-C motif) ligand 22	4.0	6.1	NS
Tgfb1	Transforming growth factor, β 1	1.6	2.2	NS

DMBA 7,12-dimethylbenz[a]anthracene HFD high saturated animal fat diet, HFD-LFD, mice on high saturated animal fat diet switched to low-fat diet, LFD low-fat diet, LFD-HFD mice on low-fat diet switched to high saturated animal fat diet, RT-PCR reverse transcription polymerase chain reaction
p < 0.05 for all genes listed, except NS = no significant change (n = 4 mice per diet treatment)

ISSN: 1465-542X