Fig. 8

Effects of diet with or without 7,12-dimethylbenz[a]anthracene (DMBA) treatment in the mammary gland at 13 weeks of age. a Proliferation in mammary glands from mice fed HFD, LFD, HFD-LFD, or LFD-HFD was measured by 5-bromo-2′-deoxyuridine incorporation. Only diet treatments with DMBA increased proliferation (*p < 0.05). b Only HFD with DMBA increased blood vessel density (CD31 staining) compared with all other diet treatments (*p < 0.05). c HFD with or without DMBA and HFD-LFD without DMBA increased macrophage recruitment (F4/80 staining) compared with LFD and LFD-HFD without DMBA. *p < 0.05. Bars represent mean ± standard error of the mean (n = 5 mice per diet and DMBA treatment). HFD high saturated animal fat diet, HFD-LFD mice on high saturated animal fat diet switched to low-fat diet, LFD low-fat diet, LFD-HFD mice on low-fat diet switched to high saturated animal fat diet