Number of patients | Target of analysis | Methods | Clinical relevance | Reference |
---|---|---|---|---|
52Â M0, 26 benign | Mitochondrial DNA quantification | qPCR | Detection of cancer | [12] |
Deregulated DNA levels | ||||
Diagnosis | ||||
51Â M0, 28Â M1, 13 benign | Nucleosome quantification | ELISA | Monitoring for therapy response | [14] |
Deregulated nucleosome levels | ||||
31Â M0, 32Â M1, 20 benign | DNA, nucleosome quantification | PicoGreen, ELISA | Detection of cancer progression | [13] |
Elevated nucleosome/DNA levels | ||||
Diagnosis | ||||
64Â M0 | DNA quantification | qPCR | Monitoring for MRD | [22] |
Inverse relationship with DTC status | ||||
100 Stage I–IV | DNA quantification | Fluorometer | Detection of cancer | [19] |
Elevated DNA levels | ||||
Diagnosis | ||||
61Â M0, 33 benign | DNA quantification | qPCR | Screening for early detection and follow-up | [20] |
Deregulated DNA levels | ||||
Diagnosis | ||||
83Â M0 | DNA integrity | qPCR | Detection of cancer progression | [24] |
Diagnosis | ||||
65Â M0, 47Â M1, 12 benign | DNA integrity | qPCR | Detection of cancer | [25] |
Diagnosis | ||||
82Â M0, 201Â M1 | DNA integrity | qPCR | Detection of cancer | [26] |
Correlation with progression-free and overall survival | ||||
Diagnosis and prognosis | ||||
65Â M0 | DNA integrity | qPCR | Monitoring for therapy response | [27] |
25Â M0 | Mutations | PCR-SSCP and direct sequencing | Detection of cancer | [5] |
Diagnosis | ||||
313Â M0 | Mutations | Digital PCR | Correlation with recurrence-free and overall survival | [33] |
Prognosis | ||||
17Â M1 | Mutations | Next-generation sequencing | Detection of metastasis | [34] |
Diagnosis | ||||
33Â M0 | Mutations | Digital PCR | Detection of cancer | [35] |
Diagnosis | ||||
30Â M1 | Mutations | Targeted sequencing | Detection of metastasis | [36] |
Monitoring for therapy response | ||||
2Â M0 | Mutations | Whole exome sequencing | Detection of acquired drug resistance in advanced cancer | [37] |
65Â M0 | SNP/CNV | Array | Detection of cancer during routine follow-up | [31] |
Correlation with MRD | ||||
Diagnosis | ||||
58Â M1 | Copy number | Whole-genome sequencing | Dynamic variation of DNA range in metastasis | [38] |
65Â M0, 58Â M1 | Copy number | Digital PCR | Screening for the acquisition of HER2 amplification in metastasis | [29] |
102Â M0, 32 benign | LOH | PCR | Correlation with overall and disease-free survival | [21] |
Diagnosis and prognosis | ||||
62Â M0 | LOH, Methylation, Mutations | Microsatellite, PCR-SSCP, MSP | Detection of tumor progression | [3] |
Diagnosis | ||||
35Â M0 | Methylation | MSP | Detection of cancer | [4] |
Diagnosis | ||||
428Â M0 | Methylation | MethyLight PCR | Correlation with overall and disease-free survival | [39] |
Therapy-independent prognosis | ||||
101Â M0, 58Â M1 | Methylation | OS-MSP | Detection of metastasis | [40] |
Diagnosis | ||||
336Â M0 | Methylation | OS-MSP | Correlation with overall survival | [41] |
Prognosis | ||||
80Â M1 | Methylation | MSP | Correlation with CTC status | [42] |
148Â M0 | Methylation | MethyLight PCR | Monitoring for therapy response | [43] |
52Â M0 | Methylation | MSP | Monitoring for therapy response | [44] |
110Â M0 | Methylation | MSP | Detection of estrogen receptor-negative status | [45] |
120Â M0, 100 benign | Methylation | MSP | Detection of cancer | [46] |
Diagnosis | ||||
155Â M0 | Methylation | MSP | Detection of metastasis | [47] |
Correlation with overall and disease-free survival | ||||
Diagnosis and prognosis | ||||
79Â M0 | Methylation | MSP | Correlation with CTC status | [48] |
Diagnosis | ||||
100Â M0 | Methylation | MSP | Correlation with protein expression | [50] |
Diagnosis | ||||
203Â M0 | Methylation | MSP | Association with DNA repair capacity | [51] |
Diagnosis | ||||
304Â M0 234 benign | Methylation | Pyrosequencing | Modest difference in methylation patterned | [52] |