Table 2 Primer sequences and methylation-sensitive high-resolution melting (MS-HRM) conditions

CCND2

F: 5′ GTTTTAGAGCGGAGAAGAG 3′

0

50

89

4

0.1 / 0.3

In-house

R: 5′ AACAAAACCTCGAAACTACC 3′

DAPK1

F: 5′ GCGCGGAGTTGGGAGGAG 3′

0

57

70

7

0.2 / 0.9

[23]

R: 5′ CTCCGAACTACCCTACCAAACC 3′

GSTP1

F: 5′ GTGAAGCGGGTGTGTAAGTTT 3′

1

56

120

12

0.9 / 3.3

[24]

R: 5′ TAAACAAACAACAAAAAAAAAACC 3′

HIN-1

F: 5′ GCGAGGATCGGGTATAAGAAGTT 3′

2

55

133

12

0.3 / 1.4

[25]

R: 5′ CACCGAAACATACAAAACAAAACCA 3′

MGMT

F: 5′ TTGATTAGGGGAGCGGTATTAG 3′

2

52

140

14

0.9 / 3.0

In-house

R: 5′ CCACATACCCGAATAATCCTAAAA 3′

RASSF1A

F: 5′ GTCGGGGTTTGTTTTGTGGTT 3′

2

56

118

9

1.5 / 5.3

In-house

R: 5′ CAACTCCCACAACTCAATAAACT 3′