Skip to main content
Fig. 9 | Breast Cancer Research

Fig. 9

From: Expression profiling of in vivo ductal carcinoma in situ progression models identified B cell lymphoma-9 as a molecular driver of breast cancer invasion

Fig. 9

BCL9 interacted with β-catenin in DCIS cell lines and BCL9 KD inhibited the Wnt/β-catenin signaling activity. a Whole-cell extracts of DCIS.COM and SUM225 cells were immunoprecipitated for BCL9 using an anti-BCL9 antibody, followed by western blot analysis using anti-β-catenin and anti-BCL9 antibody. IgG antibody was used as a negative control for IP and 2 μg of whole cell lysate was used as input. b STopFlash reporter activity in DCIS.COM non-transduced (NT), control, and BCL9-KD, that were either treated with Wnt3A or control conditioned medium (CM). c FopFlash in these cells with control or Wnt3A CM. d STopFlash reporter activity in DCIS.COM NT, BCL9-OE, constitutively active β-catenin, or all combined that were either treated with Wnt3A or control CM. e FopFlash in these cells with control or Wnt3A CM. Data represent mean ± standard error of the mean (n = 3, * p <0.05, letters indicate statistically significant difference)

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us