Fig. 7

BCL9 KD reduced the mesenchymal markers and increased the luminal markers in DCIS.COM cell line. a Representative western blot analysis of cell lysates from non-transduced (NT), control and BCL9-KD DCIS.COM and SUM225 cells blotted with anti-BCL9, anti-vimentin, and anti-E-cadherin antibodies. β-actin was used as a loading control. The labels show percent change in BCL9-KD protein compared to control. b and c show immunofluorescent images of control (a-d) and BCL9-KD DCIS.COM (e-h) xenografts stained with vimentin (b) and E-cadherin (c) (red), and K5 (green), and 4′,6-diamidino-2-phenylindole (DAPI) in (blue). Scale bars =50 μm, × 40 magnification. d Bar graphs of cell fluorescence intensity units for E-cadherin and vimentin in control and BCL9 KD DCIS.COM cells. Measurements were obtained by ImageJ. Corrected cell fluorescence was calculated by subtracting the background mean density from the total integrated density. The data represent the mean ± standard error of the mean (n = 4, *p <0.05)