Fig. 1

Differentially expressed genes in DCIS.COM and SUM225 mouse intraductal xenograft models (MIND). a The MIND model. MIND xenografts were generated by intraductal injection of DCIS.COM and SUM225 cells into the mammary ducts of immunocompromised mice. Mammary glands containing DCIS-like lesions were collected at the distinct stages of in situ to invasive lesions (2, 6 and 10 weeks) followed by digestion, magnetic sorting of epithelial cells and RNA isolation. The RNA was subjected to microarray analysis. b Heatmap of differentially expressed genes in DCIS.COM and SUM225 MIND xenografts at 2, 6 and 10 weeks. Unsupervised average-linkage hierarchical clustering of expression data from DCIS.COM and SUM225 MIND xenografts revealed that the majority of expression changes occurred at the 2-week to 6-week time point with little change occurring from 6 to 10 weeks. Further analysis was focused on the 2-week to 6-week time points. c, d Heatmap of differentially expressed genes in the canonical Wnt pathway from 2 to 6 weeks in DCIS.COM (c) and SUM225 (d) MIND xenografts. Unsupervised average-linkage hierarchical clustering was used to visualize significantly upregulated or downregulated genes in the WNT pathway, using a cutoff false discovery rate of <5 %