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Fig. 1 | Breast Cancer Research

Fig. 1

From: Expression of truncated human epidermal growth factor receptor 2 on circulating tumor cells of breast cancer patients

Fig. 1

Expression of full-length HER2 and its truncated form on control SKBR3 and SCOV3 cells. a Equal protein amounts of SCOV3 and SKBR3 cells were subjected to SDS-PAGE electrophoresis and transferred to nitrocellulose membrane. Total proteins were detected by immunoblotting (IB) with (I): anti-HER2 ECD, (II) HER2 ICD (CB11 clone) and (III) anti-actin. b Representative Ariol system images from control slides with SKBR3 cells. (a) SKBR3 cells stained with HER2 (ECD)/Alexa633/Alexa555 antibodies (b) SKBR3 cells stained with HER2 (ICD)/Alexa633/Alexa555 antibodies and (c) SKBR3 cells stained with HER2(ECD)/HER2 (ICD)/Alexa633/Alexa555 HER2 antibodies. c Representative confocal laser scanning micrographs (×40) of SKBR3 and SCOV3 cells triple-stained with HER2 (ICD), HER2 (ECD) and pancytokeratin (A45-B/B3) antibodies. ECD and ICD were identified in SKBR3 (A-D) cells while low (E-H) or no ECD (I-L) was identified in SCOV3 cells. ECD extracellular domain, HER2 human epidermal growth factor receptor 2, ICD intracellular domain

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