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Fig. 4 | Breast Cancer Research

Fig. 4

From: Leptin produced by obese adipose stromal/stem cells enhances proliferation and metastasis of estrogen receptor positive breast cancers

Fig. 4

Leptin inhibition reduces the expression of key regulatory genes involved in invasion and metastasis. Breast cancer cells (BCCs) were co-cultured CC with adipose stromal/stem cells isolated from lean women (lnASCs) or adipose stromal/stem cells isolated from obese women (obASCs) stably transfected with a control short hairpin RNA (control shRNA) or leptin shRNA. After 7 days, co-cultured cells were sorted by FACS and BCCs were collected for total RNA extraction and cDNA synthesis. Genes involved in invasion and metastasis were assessed. Bar represents ± standard error of the mean. *P <0.05, ***P <0.001 relative to BCCs cultured alone; ### P <0.01 for comparison between BCCs after co-culture with control shRNA lnASCs and control shRNA obASCs; ΦΦΦ P <0.001 for comparison between BCCs after co-culture with control shRNA obASCs and leptin shRNA obASCs. MMP matrix metalloproteinase

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