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Fig. 3 | Breast Cancer Research

Fig. 3

From: Human breast cancer cells educate macrophages toward the M2 activation status

Fig. 3

Cytokine secretion profile after 24 h of lipopolysaccharide (LPS)-stimulation. a-c CD14+ cells differentiated for 5 days with or without 50 % breast cancer cell line conditioned media (CM) (d-f) in the presence of IL-4 (g-i) in the presence of IL-10. a-i All results are the mean of three experimental replicates and two biological replicates. j Representative matrix metalloproteinase (MMP)-9 zymography gel (k) relative MMP-9 activity, expressed as digested band optical density normalized to equivalent background area optical density. Error bars represent + SD, n = 3, *p <0.05, **p <0.005, ***p <0.0005 (Kruskal-Wallis analysis followed by Dunn’s post hoc test). MB231, MDA-MB231 CM; NT, non-treated

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